Protein Method Blocking Buffers
ILEX LIFE SCIENCES LLC Block Ace Powder: blocking reagent for immunological experiments (1 x 40 g)
Block Ace Powder: blocking reagent for immunological experiments. Block Ace is a blocking agent prepared from bovine milk proteins (lactoprotein). Since lactoprotein contains a lot of hydrophobic groups, it shows a high affinity to plastic, membrane filter, etc. Block Ace exhibits a superior blocking effect on nonspecific adsorption of antigens or antibodies in ELISA, RIA and immunoblocking methods compared with conventional blocking agents such as BSA (Bovine Serum Albumin). FEATURES: Since Block Ace has high blocking effects and maintains the background at a low level, it is useful for low-concentration measurement such as ELISA. Block Ave is heat-treated and thus bovine-derived antibodies, enzymes and viruses are inactivated. Block Ace is stable for heat and freezing. It can stabilize physiologically active substances and can be used as a diluting agent. Block Ace can be rapidly dissolved in water. MANUFACTURER: KAC Co., Ltd., Kyoto-city 603-8432, Japan.
LI COR BIOTECH LLC Intercept (PBS) Protein-Free Blocking Buffer (3x500mL)
Intercept (PBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. This buffer contains no proteins, for assays where use of animal-based products is prohibited. They are an excellent choice for biotin-streptavidin detection and suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in phosphate-buffered saline and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
IBI SCIENTIFIC 3P 10X PBST (PBS W/0.5% TWEEN 20) 4/PACK
Sterile buffer solution containing sodium phosphate, sodium chloride, potassium phosphate and potassium chloride with 0.5% TWEEN 20.Sterile filtered using 0.1um filterDNase, RNase and protease free.Sterility validated using USP <71> Test for SterilityDoes not contain calcium, magnesium, phenol red or sodium pyruvate. IBI 10X PBST (10X PBS) with TWEEN 20 (0.5% TWEEN 20) is a concentrated sterile solution in a 1 liter bottle that is ideal for use in immunoassay procedures such as Western Blotting and ELISAs in microtiter plate wells.IBI 10X PBST is mixed and bottled in our liquid production facility here in Dubuque, Iowa. Our high output pure water system produces endotoxin free and nuclease free water at a rate of 100 gallons per/hr. This water is the basis for all of our liquid blending production here at IBI.
SCANTIBODIES LABORATORY INC Heterophilic Blocking Tube
This item must be ordered in multiples of 50 per supplier requirements
Tube, Blocking; Heterophilic; Unique blocking reagent composed of specific binders which inactivate heterophilic antibodies; Allows rapid and simple elimination of false positive heterophilic interference in plasma for sandwich immunoassays.
Sigma Aldrich Fine Chemicals Biosciences Gelatin blocking buffer for Western blotting, powder blend | 1L
Gelatin blocking buffer for Western blotting, powder blend | 1L
LI COR BIOTECH LLC Intercept (PBS) Protein-Free Blocking Buffer (500 mL)
Intercept (PBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. This buffer contains no proteins, for assays where use of animal-based products is prohibited. They are an excellent choice for biotin-streptavidin detection and suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in phosphate-buffered saline and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
Cell Biolabs Inc PhosphoBLOCKER™ Blocking Reagent, 200 g dry blend (4 Liters prepared)
The utilization of anti-phosphoprotein antibodies in western blotting has become a commonly used tool for signal transduction research. Unfortunately, low levels of endogenous phosphoprotein in various cell lysates often can not be detected, even with high concentrations of antibody and long exposure times. Most commercially available western blot blockers (e.g. dry milk, serum) are sufficient to block the unreacted sites on the membrane, reducing the amount of nonspecific antibody binding during the assay; however, they are not designed to preserve phosphoprotein antigens during blotting. Cell Biolabs’ PhosphoBLOCKER™ contains a proprietary formulation that provides several advantages over conventional blockers: (1) It is designed specifically for phosphoprotein blotting; (2) It enhances low level phosphoprotein signal without increasing background; and (3) it is an easy to use premixed dry blend. 200 grams (reconstituted to 4 liters).
Teknova 0.01M PBS with 0.02% Tween-80. 1000mL, Sterile
0.85% Sodium Chloride, 0.12% Sodium Phosphate Dibasic, 0.022% Sodium Phosphate Monobasic, 0.02% Tween-80
LI COR BIOTECH LLC Intercept (TBS) Protein-Free Blocking Buffer (500 mL)
Intercept (TBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays where animal-based products are prohibited. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no proteins, for assays where animal-based products are prohibited, it can be used with the widest range of targets for Western blot detection, it is ideal for primary antibodies that are optimized for Tris-based buffer systems, it can be used for detection of phosphorylated targets, it has superior performance for alkaline phosphatase detection, and it is an excellent choice for biotin-streptavidin detection. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in TBS and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
Research Products International Corp BSA-Tween 20 Blocking Solution in TBS, 500 Milliliters
Contains 3% BSA and 0.05% Tween 20 in TBS buffer. Optimized for use in western blotting to minimize non-specific binding of antibodies.
Research Products International Corp Casein Blocking Solution in TBS, 1 Liter
Contains 3% Casein in TBS buffer. Optimized for use in western blotting to minimize non-specific binding of antibodies.
Seracare Life Sciences Inc 10X DULBECCO S PBS W/ TWEEN 20
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications as well as tissue and cell culture procedures. This modified Dulbecco's buffer is not formulated with magnesium or calcium salts. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures. pH=7.4. Conductivity controlled.
Seracare Life Sciences Inc 10% BSA DILUENT/BLOCKING SOL
Convenient antibody diluent/blocking solution concentrate containing 10% bovine serum albumin in phosphate buffer. Compatible with most assay formats.